Extracellular Pectinolytic Enzymes of Fungi Elicit Phytoalexin Accumulation in Carrot Suspension Culture

Carrot Suspension Culture Cells

Host-Pathogen Interactions XV. FUNGAL GLUCANS WHICH ELICIT PHYTOALEXIN ACCUMULATION IN SOYBEAN ALSO ELICIT

A 8jglucan isolated from the mycelial wails of Phytophthora megasperma var. sojae and a glucan purified from yeast extract stimulate the accumulation of phytoalexins in red kidney bean, Phaseolus vulgads, and stimulate the accumulation of the phytoalexin, rishitin, In potato tubers, Solanum tuberosum. These glucans have previously been shown to be potent elicitors of glyceollin accumulation In ...

Synchronization of somatic embryogenesis in a carrot cell suspension culture.

Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were compose...

Potassium transport in suspension culture cells and protoplasts of carrot.

The properties of potassium transport in carrot (Daucus carota L.) suspension culture cells and their isolated protoplasts were examined. Cells cultured in Murashige and Skoog (MS) medium (Plant Physiol 15: 473-497) were potassium saturated and, consequently, they exhibited little net potassium accumulation. Cells that transport and accumulate potassium were derived from the MS-grown cells by c...

Differential Accumulation of Biotin Enzymes during Carrot Somatic Embryogenesis.

The activities of four biotin enzymes, acetyl-coenzyme A (CoA) carboxylase, 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase, and the accumulation of six biotin-containing polypeptides were determined during development of somatic embryos of carrot (Daucus carota). Acetyl-CoA carboxylase activity increased more than sevenfold, whereas the activities of 3-met...